【摘要】 目的　充分发挥脊灰病毒学监测作用。方法　按照《脊灰病毒检验手册》操作规程进行病毒分离与鉴定,由国家脊灰实验室用PCR -RFLP法对分离到的脊灰病毒株做型内鉴别。结果2 0 0 3年河北省脊灰实验室各项监测指标仍然保持较高水平。从AFP病例粪便标本中分离到脊灰病毒(PV) 4 3株,分离率为11.6 2 % ;非脊灰肠道病毒(NPEV) 4 9株,分离率为13.2 4 %。所有PV经国家脊灰实验室进行型内特征鉴定,除2株为疫苗变异株外,其余均为疫苗相关株。结论　三种细胞可以提高PV分离率,RD和HEp - 2细胞对提高NPEV的分离率都是很重要的。NPEV的分离率与月份有关。更多还原

【Abstract】 Objective This analysis was designed to make use of the monitoring function of virology. Methods Viruses were isolated and identified according to operational regulations in Testing Manual for Poliovirus, and PCR-RFLP was adopted to identify the isolated type of virus strains at National Poliomyelitis Laboratory. Results Each lab monitoring index of poliomyelitis remained at a high level in Hebei of 2003. 43 strains of poliomyelitis virus (PV) were isolated from AFP feces samples, with a rate of 11.62%, in addition to 49 strains of non-poliomyelitis enterovirus (NPEV), with an isolation rate of 13.24%. All PVs were sent for typing identification at National Poliomyelitis Laboratory, the result of which showed that except two vaccine variant strains, others were all vaccine-related strains. Conclusion Three cells can improve the isolation rate of PV. Moreover, both RD and HEp-2 cells are important for enhancing the isolation rate of NPEV which has relevance with months.更多还原