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外源钙对柑橘抗热性的相关生理生化指标的影响

Effects of Exogenous Calcium on the Correlative Physiological and BiochemicalIndex on Heat-resistance of Citrus

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【作者】 张建霞李新国孙中海

【Author】 Zhang Jianxia Li Xinguo Sun Zhonghai(Citrus Research Institute, Huazhong Agricultural University, Wuhan 430070,China)

【机构】 华中农业大学柑橘研究所华中农业大学柑橘研究所 武汉430070武汉430070武汉430070

【摘要】 以柑橘品种国庆1号温州蜜柑(CitrusreticulataBlancocv.GuoqingNo.1)、朋娜脐橙(C.sinensisOsbeckcv.SkaggesBonnaza)及葡萄柚(CparadisiMacq)叶片为材料,研究了外源钙预处理对高温胁迫下可溶性糖、可溶性蛋白质含量、SOD活性及MDA含量的影响。结果表明:高温胁迫下,可溶性糖含量随外源Ca2+浓度的增加而增加;可溶性蛋白质含量和SOD活性都呈先升后降的趋势,当外源Ca2+浓度为20mmol/L时,可溶性蛋白质含量最高;当外源Ca2+浓度为10mmol/L时,SOD活性最高。3个品种对外源Ca2+敏感度差异较大,SOD活性越高,对Ca2+敏感度越低,增加率越低,其相关系数r=-0.9966。高温胁迫下,3个品种的MDA含量差异显著,随外源Ca2+浓度的增加,MDA含量呈先降后升的趋势,Ca2+浓度为20mmol/L时,含量最低。以上结果都说明适量的Ca2+预处理可增强柑橘的抗热性。

【Abstract】 Citrus leaves were used to examine the effect of exogenous Ca 2+ pretreatment on the activity of SOD and the contents of soluble sugar, soluble protein and MDA. The results showed that under high temperature stress, the content of soluble sugar was distinctly different among varieties, which tended to rise with the increasing of sprayed Ca 2+ concentration. The content of soluble protein and the activity of SOD tended to rise first, and then drop. When Ca 2+concentration was 10 mmol/L, SOD activity reached the maximum. Whileprotein content reached the maximum when Ca 2+concentration was 20mmol/L. When leaves of the citrus were sprayed with exogenousCa 2+, the sensitivity of different varieties to heat stress was clearly different using SOD as parameter. The higher SOD activity, the lower sensitivity citrus responded to it and the smaller increasing rate. The correlative coefficient was - 0.9966. Under hightemperature stress,MDA contentwas distinctly different among varieties. It dropped first, and then rise with the increasing of Ca 2+ concentration.When Ca 2+concentration was 20mmol/L, MDA content reached the minimum. These results showed that proper Ca 2+ might strengthen citrus heat-resistance.

【关键词】 柑橘Ca2+抗热性
【Key words】 citruscalcium ionheat-resistance
【基金】 国家自然科学基金项目(30070528)资助
  • 【文献出处】 华中农业大学学报 ,Journal of Huazhong Agricultural , 编辑部邮箱 ,2005年04期
  • 【分类号】S666
  • 【被引频次】36
  • 【下载频次】437
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