【摘要】 cDNA -AFLP结合了RFLP和RT- PCR的优点,在无需了解序列信息的同时,集中显示基因组表达序列的多态性差异,灵敏性高,重复性好。但实验室开展常规的cDNA- AFLP研究需要昂贵的设备和大量的投入。本试验改用小面积变性聚丙烯胺凝胶电泳检测扩增产物,银染显带法来完成cDNA- AFLP的全套技术体系,使cDNA -AFLP分析方法更为简便、经济并减少污染。更多还原

【Abstract】 cDNAAFLP combines the strongpoint of RFLP and RTPCR into a useful technique for the study of gene expression and transcript profiling. Because of its high reproducibility, accuracy and reliability,it is widely applied to analyse comprehensively and systematically the transcriptome.But it will cost a lot of money to buy equipment to accomplish cDNAAFLP. The MINIPR OTRAN gel electrophoresis of BIORED Company was used to optimize cDNAAFLP with silver staining which make it unite the advantages of low cost and less pollution.更多还原