【摘要】 目的探讨回转模拟微重力对人的血管内皮细胞形态、NO产生及一氧化氮合酶表达的影响。方法利用体外培养的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC),采用回转器模拟微重力效应,在微重力条件下培养人脐静脉内皮细胞48h,同时设1g静止培养对照。倒置显微镜下观察细胞生长形态,透射电镜观察HUVEC超微结构。检测不同转速下细胞产生NO的量,及细胞中内皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS)的表达。结果微重力培养后,细胞变圆且重叠生长;回转组NO产生量高于1g对照组且与一定的回转速度呈正相关。回转组细胞eNOS表达较对照组增多且表达iNOS,对照组细胞未表达iNOS。结论微重力对体外人脐静脉内皮细胞的形态,NO产量及NOS的合成都有影响。更多还原

【Abstract】 Objective To investigate the morphology, production of nitric oxide (NO) and the expression of nitric oxide synthase of human umbilical vein endothelial cells(HUVEC) under the condition of microgravity.Method The HUVEC cultured in flasks on microgravity-based clinostat for 48 h were the microgravity group,and those cultured in flasks under 1 g condition were control group.Morphology of the cells were observed by inversion microscope and transmission electron microscope. The flasks of the microgravity group were centrifuged at different rates , the supernatant was harvested and the production of nitric oxide and the endothelial nitric oxide synthase (eNOS) and nitric oxide synthase (iNOS) were assayed. Result The morphology and ultrastructure of HUVEC cultured under microgravity changed.The cells became round and growed overlapingly. NO production of the cells increased significantly , and was positivly related with the degree of microgravity;eNOS expression increased and iNOS expression of the cells cultured under microgravity was observed. Conclusion Microgravity can influence the morphology, ultrastructure, production of NO, and the expression of nitric oxide synthase of HUVEC in vitro.更多还原