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乙酸对两种杜氏藻生长和细胞生化组成的影响

Effects of acetic acid on the growth and chemical composition of two species of Dunaliella

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【作者】 王培磊张学成孟振张积军

【Author】 WANG Pei-lei, ZHANG Xue-cheng, MENG Zhen, ZHANG Ji-jun (College of Marine Life, Ocean University of China, Qingdao 266003, China;Inner Mongolia, Lantai Co. LTD, Alashan 750333, China)

【机构】 中国海洋大学海洋生命学院内蒙古兰太实业股份有限公司生物分公司 山东 青岛 266003山东 青岛 266003内蒙古 阿拉善盟 750333

【摘要】 研究了乙酸对两种杜氏藻(Dunaliella salina和D.parva)生长、β-胡萝卜、素积累、叶绿素a合成、脂肪酸组成和蛋白质含量的影响。结果表明,对D.salina,乙酸可明显促进生长。细胞密度最大值120×104cell/mL(pH≤8.5),β-胡萝卜素最大值102mg/g(pH≤8.0),叶绿素a含量达到104 mg/g(ph≤8.5),与对照组相比均有显著差异;乙酸还可提高单不饱和脂肪酸18. 1和多不饱和脂肪酸18:2n6的含量;蛋白含量随pH升高而提高。对D.parva,乙酸对生长无明显促进作用,也不能提高β-胡萝卜素含量,但明显提高叶绿素a含量,最大值达144 mg/g (pH≤9.0),还可提高蛋白含量,达到33.5%(ph≤9.0)。

【Abstract】 The effects of acetic acid on the growth and chemical composition of two species of Dunaliella were investigated. Acetic acid can significantly enhance the growth rate of D. salina. The maximum cell density of 120 ×104 cell/mL, β - carotene content of 102 mg/g and Chl a content of 104 mg/g were observed in treated groups of pH≤8.5, pH≤8.0 and pH≤8.5, respectively. Three of than significantly differed from those of the control group. Acetic acid also enhanced both the contents of monounsaturated fatty acid of 18 : 1 and the content of polyunsaturated fatty acid of 18 : 2n6. The higher the pH values, the higher the contents of protein. To D. parva, acetic acid was found neither to enhance the growth rates, nor to enhance the contents of β-carotene of the culture media, though it was found to enhance both the content of Chl a, reaching 144 mg/g (pH≤9.0), and the content of protein, reaching 335 mg/g (pH≤9.0).

【基金】 国家863计划(863-819-04-10)
  • 【分类号】Q945
  • 【被引频次】9
  • 【下载频次】139
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