【摘要】 目的观察槲寄生黄酮苷对大鼠心室肌细胞钾离子通道的作用,探讨槲寄生黄酮苷抗心律失常作用机制。方法应用全细胞膜片钳技术记录槲寄生黄酮苷对大鼠单个心室肌细胞内向整流钾电流(IK1)、瞬时外向钾电流(Ito)的影响。结果应用50μg/ml和250μg/ml两种浓度的槲寄生黄酮苷分别使大鼠单个心室肌细胞内向整流钾电流(IK1)在实验电压-120mV时从给药前(-26.23±9.52)pA/pF减少到(-20.82±7.88)pA/pF,(-18.11±7.89)pA/pF(n=7,P<0.05);使大鼠心室肌细胞瞬时外向钾电流(Ito)在实验电压+50mV时,从给药前(26.14±6.67)pA/pF减少到(16.41±6.23)pA/pF,(13.25±3.78)pA/pF(n=4,P<0.05)。结论槲寄生黄酮苷抑制大鼠心室肌细胞IK1,Ito可能是其抗心律失常作用的一个机制。更多还原

【Abstract】 Objective To investigate the effect of viscum coloratum flavonoid (VCF) on inwardly rectified potassium current(I K1 ) and transient outward potassium current (I to ) in isolated rat ventricular myocytes.Methods Whole cell patch clamp technique was used.Results With calcium current blocked by CdCl 2 at 0.3 mmol/L,VCF inhibited I K1 and I to .VCF at 50 and 250μg/ml deceased I K1 from (-26.23±9.52)pA/pF to (-20.82±7.88)pA/pF,and (-18.11±7.89)pA/pF ( n =7, P <0.05),respectively and inhibited peak I to from (26.14±6.67)pA/pF to (16.41±6.23)pA/pF,and (13.25±3.78)pA/pF ( n =4, P <0.05),respectively.Conclusion VCF may have antiarrhythmic effect because it can inhibit potassium currents.更多还原