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紫外分光光度法测定川白芷中总香豆素类成分的含量
Determination of total content of coumarin in Angelica dahurica by UV spectrophotometry
【摘要】 目的 建立川白芷药材中总香豆素的含量测定方法。方法 采用紫外分光光度法,测定波长30 0nm。结果 线性范围2 .2~11.0 μg·ml-1(r=0 .9999) ,回收率为10 1.32 % ,RSD =3.34% (n =6 )。结论 所用方法准确、快速、重复性好,可用于川白芷药材中总香豆素的测定。
【Abstract】 OBJECTIVE To determine the total content of coumarin in Angelica dahurica. METHODS The Coumarin in Chuan Baizhi was detected at 300 nm by UV method.RESULTS There was a good linear relationship within the range of 2.2-11.0 μg·ml -1 (r=0.9999).The average recovery was 101.32%(n=6) with RSD of 3.34%. CONCLUSION This method is rapid, reproducible, accurate and can be used for measuring the total content of coumarin in Chuan Baizhi.
【关键词】 川白芷;
总香豆素;
紫外分光光度法;
含量测定;
【Key words】 Angelica dahurica; Total content of coumarin; UV Spectrophotometry; Content determination;
【Key words】 Angelica dahurica; Total content of coumarin; UV Spectrophotometry; Content determination;
【基金】 四川省科技厅资助项目 ,课题名称 :川白芷规范化种植 (GAP)的研究。
- 【文献出处】 华西药学杂志 ,West China Journal of Pharmaceutical Sciences , 编辑部邮箱 ,2005年02期
- 【分类号】R284
- 【被引频次】90
- 【下载频次】1250