【摘要】 不同浓度化学修饰剂二巯基苏糖醇(DTT)、对氯汞苯甲酸(pCMB)、N 溴代琥珀酰亚胺(NBS)、苯甲基磺酰氟(PMSF)、溴代乙酸(BrAc)的修饰能抑制瘦肉型猪(PIC344)精液酸性磷酸酶(ACPase,E C 3 1 3 2)的活力。同一浓度不同种类的修饰剂修饰酶活力降低程度不同。化学修饰剂修饰ACPase不同时间后,酶活力均有不同程度的降低,其中PMSF作用60min后酶活力仅存18%,而BrAc的修饰对ACPase的活力无明显影响。更多还原

【Abstract】 Acid Phosphatase(ACPase,E.C.3.1.3.2) was modified by NBS,PMSF,pCMB,DTT and AcBr.The effects of differert concentration and time on the ACPase catalytic activities were determined.The reactions of ACPase from PCI344 with NBS,PMSF,pCMB and DTT resulted in a strong inhibition of enzyme activities.The activities of ACPase decreased steadily with the increase of modification reagents concentration and time.Histidine residues modified by BrAc did not change the activity of the enzyme,while the activity only remained 18%after PMSF modified ACPase for 60min.更多还原