【摘要】 采用戊二醛法和羰基二咪唑法,将半抗原氯霉素(CAP)与载体牛血清白蛋白(BSA)偶联,制得氯霉素全抗原CAP BSA,并由紫外光谱和红外光谱确定偶联成功。所合成抗原的聚丙烯酰胺凝胶电泳(SDS)只见一条区带,表明所合成的氯霉素与BSA偶联物可以作为免疫抗原。用市售的氯霉素快速检测试剂盒可以定量测定所合成抗原中氯霉素的含量。将固体配成1mg mL溶液,用TNBS法测氨基酸残基,得到BSA上偶联的氯霉素数目。更多还原

【Abstract】 In order to establish an enzyme-linked immunosorbent assay (ELISA) for rapidly examing residual chloramphenicol (CAP) in food, the antibody with high titer and specificity must be firstly prepared. So it was necessary for antibody production to conjugate the hapten (CAP) with a carrier protein to synthesize immunogen (antigen). Chloramphenicol was coupled with carrier protein bovine serum albumin (BSA) to prepare complete antigen CAP-BSA by glutaraldehyde (GA) and (N,N′)-carbonyldiimidazole (CDI). The successful linkage of the CAP-BSA was identified by UV and IR. There was only one zone in SDS-PAGE and the products could be considered as the immunogen. An ELISA kit had been used to determine the content of CAP in the CAP-BSA. The residual amount of amino groups was determined by the TNBS spectrometry, and the amount of CAP linked to BSA was achieved. All the above results indicated that the immunogen CAP-BSA was successfully synthesized, which was going to be injected in the animals to obtain CAP antibody.更多还原