【摘要】 目的　观察膜联蛋白I(AnnexinI)和胞浆磷脂酶A2(cPLA2)在地塞米松致畸敏感性小鼠腭胚中的分布情 况。方法　沿小鼠胚胎头部的冠状平面切取切片,获得10周龄近交系小鼠妊娠的第14～16天的腭胚突。用免疫 组化染色检测AnnexinI和cPLA2的分布情况。结果　在小鼠胚胎腭突融合前后,AnnexinI、cPLA2的免疫组织化学 染色在腭突上皮和间充质细胞中都为阳性,并且染色强弱呈时相性变化。结论　AnnexinI和cPLA2在胚胎腭部发 育进程中起一定的调节作用,可能是地塞米松诱导发生腭裂的重要介质。更多还原

【Abstract】 Objective To observe the distribution of Annexin I and cytosolic phospholipose A₂ （cPLA₂） in the palatal process of dexamethasome teratogenerated mice.Methods Cutting along the coronary plane of the heads of fetal mice to obtain palate in the 14th, 15th, 16th day of gestation by 10-week-old in-bred mice. The distribution of Annexin I and cPLA₂ was checked by immunohistochemical staining.Results During the fusing of the palatal processes, the staining of Annexin I and cPLA2 was positive in the epithelial and mesenchymal cells of the palatal process, and the intensity of staining changed with time.Conclusion Annexin I and cPLA₂ can modulate the development of fetal palate to some extent, and they may be important mediators in the development of cleft palate induced by dexamethasone.更多还原