【摘要】 该文研究了以硼掺杂金刚石为基底电极的酪氨酸酶传感器。该酶传感器对酚的催化作用强于以玻碳为基底电极的酪氨酸酶传感器。在浓度为1.0×10^-8～1.0×10.^-5mol/L 的范围内传感器对邻苯二酚的响应具有良好的线性关系,检测下限为5.2×10^-9mol/L。酶电极的 Michaelis～Metent 常数(K_m^app)为33.65μmol/L。酶电极对苯酚和对甲苯酚也有良好的响应,线性范围分别为:5.0×10^-8～2.0×10^-5～mol/L、5.0×10^-8～5.0×10^-6～mol/L。酶传感器有较好的稳定性和重现性。更多还原

【Abstract】 An tyrosinase biosensor based on a boron-doped diamond （BDD） electrode as the base electrode has been developed.The enzyme biosensor catalyzes phenolic compounds to get quinines which can be reduced to produce re- duction current. The catalysis to phenols of such biosensor is better than the one which was based on the glassy carbon electrode as the base electrode.The enzyme electrode provided a linear response to cateehol over a concentration range of 1.0×10^-8 to 1.0×10^-5 mol/L with a detection limit of 5.2×10^-9 mol/L.The apparent Michaelis-Menten constant (K_m^app) for the sensor was calculated to be 33.65μmol/L.The enzyme biosensor has good responses to phenol and p-cresol,the linear calibration ranges to them are 5.0×10^-8～2.0×10^-5 mol/L and 5.0×10^-8～5.0×10^-6 mol/L respectively.The biosensor was evaluated with satisfactory analytical performance in terms of the repeatability and stability.更多还原