【摘要】 以 ×L-15 培养基为基础,添加不同比例的胎牛血清和对虾肌肉提取液配制成不同的培养液,当 22×L-15培养基、胎牛血清和对虾肌肉提取液的比例分别为 60 %、20 %、20 %时,培养效果较好。培养液 pH 维持在7.0 ~ 7.2时血淋巴和类淋巴细胞生长最好,且类淋巴细胞迁出迅速。绝大部分血淋巴细胞贴壁迅速并伸展成多角样或披针形,一般存活 7 ~ 9 d,少部分呈悬浮状态的血淋巴细胞存活达 60 d 以上。培养中如未及时去除对虾血清,血淋巴细胞迅速衰老并死亡。类淋巴组织细胞 6 d可形成单层,迁出细胞主要有两种形态: 多角样和成纤维样,细胞可存活 16 d以上。更多还原

【Abstract】 Primary cell culture from hemolymph and lymphoid tissues of shrimp,Litopenaeus van namei was carried out in this article. Culture media was based on 2×L-15 medium, supplemented with fetal bovine serum (FBS) and shrimp muscle extract (SME) at different proportions. The result of cell culture was better when the proportions of 2 ×L-15 medium, FBS and SME are 60 %, 20 % and 20 %, respectively. Cells from hemolymph and lymphoid tissues grew best and lymphoid cells emigrated quickly when pH of the media was kept at the range from 7.0 to 7.2. Most of hemolymph cells attached fast and stretched into multiangular or acerose shape with an average life span of 7-9 days. Few round cells suspended and survived over 60 days. In the hemolymph cell culture, cells would age and die soon unless shrimp serum was removed . Lymphoid cells formed a single layer within 6 days and they were mainly classified into two types according to the attaching shape: multiangular and fibroid. These cells could survive more than 16 days.更多还原