【摘要】 目的研究银杏叶提取物EGb761对肿瘤坏死因子—α(TNF—α)诱导HeLa细胞凋亡和Caspase—8活性的影响。方法采用流式细胞术检测细胞凋亡,Caspase—8荧光检测试剂盒测定Caspase—8活性。结果EGb761在10~40 mg/L终浓度范围内对rhTNF—α诱导的HeLa细胞凋亡均有显著的抑制作用(P<0.01),呈剂量依赖关系;EGb761在10~40 mg/L终浓度范围内对rhTNF—α诱导的HeLa细胞Caspase—8活化均有显著的抑制作用(P<0.01),呈剂量依赖关系。结论结果提示,EGb761抑制TNF—α诱导的HeLa细胞凋亡可能与其抑制Caspase—8活化有关。更多还原

【Abstract】 Objective To investigate the effects of Ginkgo biloba extract EGb761 on apoptosis of HeLa cell induced by tumor necrosis factor-α.(TNF—α.) and on the activity of Caspase—8. Methods The effect of EGb761 on the apoptosis of HeLa cell was induced by rhTNF—α and Caspase—8 fluorescent kits was used to detect the activity of Caspase-8. Results The result of flow cytometry exhibited that EGb761 with 10～40mg/L final concentration had notable inhibition but not absolute inhibition on the apoptosis of HeLa cell induced by rhTNF—α (P<0.01)and it showed dose-dependence. The detection of the activity of Caspase—8 showed that EGb761 with 10～40mg/L final concentration also had notable inhibition on the Caspase—8 activation of HeLa cell induced by rhTNF—α (P<0.01). Conclusion The inhibition of apoptosis in HeLa cell induced by rhTNF—α by EGb761 may have relation to the Caspase—8 activation.更多还原