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HPLC法测定小儿增食片中大黄素和大黄酚的含量

Determination of the Content of Emodin and Chrysophanol in Xiaoer Zengshi Pills by HPLC

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【作者】 江行本高超旭张志远

【Author】 Jiang Xingben~1,Gao Chaoxu~2,Zhang Zhiyuan~3 Suiyang Institute for Drug Control of Shangqiu, Henan Province; Yikang Institute of Materia Medica of Zhengzhou Hi-tech Industries Development Zone;Zhengzhou College of Animal Husbandary Engineering

【机构】 河南省商丘市睢阳区药检所郑州市高新区意康药物研究所郑州牧业工程高等专科学校

【摘要】 目的:用高效液相色谱法测定小儿增食片中大黄素和大黄酚的含量。方法:固定相为BDS柱,流动相为甲醇-0.1%磷酸(85:15),检测波长254nm。结果:回收率为99.52%,RSD值3.04%。结论:该法能使干扰峰与大黄素和大黄酚峰完全分离。

【Abstract】 Objective: To determine the content of emodin and chrysophanol in Xiaoer Zengshi pills with HPLC. Method: BDS column was taken as the fixed phase and methanol and 0.1% phosphoric acid(85∶15) as the mobile phase. The detection wavelength was 254 nm. Result: The rate of recovery was 99.52% and RSD value was 3.04%. Conclusion: The method can separate interference peak from emodin and chrysophanol peak.

【关键词】 大黄素大黄酚高效液相色谱法小儿增食片
【Key words】 emodinchrysophanolHPLCXiaoer Zengshi Pills
  • 【文献出处】 河南中医学院学报 ,Journal of Henan College of Traditional Chinese Medicine , 编辑部邮箱 ,2005年03期
  • 【分类号】R286.0
  • 【被引频次】1
  • 【下载频次】61
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