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HPLC法测定小儿增食片中大黄素和大黄酚的含量
Determination of the Content of Emodin and Chrysophanol in Xiaoer Zengshi Pills by HPLC
【摘要】 目的:用高效液相色谱法测定小儿增食片中大黄素和大黄酚的含量。方法:固定相为BDS柱,流动相为甲醇-0.1%磷酸(85:15),检测波长254nm。结果:回收率为99.52%,RSD值3.04%。结论:该法能使干扰峰与大黄素和大黄酚峰完全分离。
【Abstract】 Objective: To determine the content of emodin and chrysophanol in Xiaoer Zengshi pills with HPLC. Method: BDS column was taken as the fixed phase and methanol and 0.1% phosphoric acid(85∶15) as the mobile phase. The detection wavelength was 254 nm. Result: The rate of recovery was 99.52% and RSD value was 3.04%. Conclusion: The method can separate interference peak from emodin and chrysophanol peak.
- 【文献出处】 河南中医学院学报 ,Journal of Henan College of Traditional Chinese Medicine , 编辑部邮箱 ,2005年03期
- 【分类号】R286.0
- 【被引频次】1
- 【下载频次】61