【摘要】 目的评估腺病毒介导的转BMP-2基因的同种异体骨髓基质干细胞的成骨潜能和免疫原性,探索骨缺损修复新途径。方法将编码骨髓基质干细胞的基因,通过腺病毒介导转染同种异体骨髓基质干细胞后复合藻酸钙凝胶植入骨缺损部位并应用免疫抑制剂FK506。结果术后8周检查,Adv-hBMP-2转染的同系骨髓基质干细胞-藻酸钙复合体组、Adv-hBMP-2转染的同种异体骨髓基质干细胞-藻酸钙-FK506复合体组以板层骨为主;Adv-hBMP-2转染的同种异体骨髓基质干细胞-藻酸钙复合体组以编织骨和类骨质为主,内有炎症细胞浸润;Adv-βgal转染的同系骨髓基质干细胞-藻酸钙复合体组、Adv-βgal转染的同种异体骨髓基质干细胞-藻酸钙-FK506复合体组、Adv-βgal转染的同种异体骨髓基质干细胞-藻酸钙复合体组无明显新骨形成,缺损区内为纤维结缔组织。结论Adv-hBMP-2转染同种异体骨髓基质干细胞-藻酸钙-FK506复合体可促进长骨干节段性骨缺损的修复。更多还原

【Abstract】 Objective To evaluate the effectiveness of adenovirus mediated human bone morphogenetic protein-2 gene transferred allogenic mesenchymal stem cells in the repair of segmental bone defect of long bone and their immunogenicity. Methods The technique involves obtaining transferred allogeneic rat MSCs and implanting them to the defect with calcium alginate gel and using immunosuppressant Fk506. Results Specimens were harvested at 8th week after operation. Histological analysis demonstrated that the defects in syngeneic MSC/Adv-hBMP-2 group and allogeneic MSC/Adv-hBMP-2 with FK506 group were filled with coarsed trabecular bone. The defects in allogeneic MSC/Adv-hBMP-2 without FK506 group were filled with woven bone or preossous tissue and were infiltrated by inflammatory cells. In the other three groups(syngeneic MSC/Adv-βgal and allogeneic MSC/Adv-βgal with or without FK506) demonstrated little or no bone formation and the defects were filled with connective tissue. Conclusion Adenovirus mediated BMP-2 gene transferred allogeneic rat mesenchymal stem cells can repair segmental defects of long bones if the host receives short-term treatment with immunosuppressant FK506.更多还原