【摘要】 【目的】调查华人、马来人及印度人的白介素 4 基因多态性与哮喘和哮喘严重度是否有相关性。【方法】研究人群包括315名哮喘患者(华人 152,马来人 76,印度人 87) 和 360 名无哮喘病史的健康献血者(华人157,马来人100,印度人103) 。患者组进一步分为轻、中度哮喘组(FEV1>=60%预计值) 和重度哮喘组(FEV1<60%预计值) 。白介素4基因启动子(C 590T) 通过 PCR限制性扩增片段长度多态性(PCR RFLP)方法检测。统计学分析应用多元回归法检测白介素基因与哮喘的关系。【结果】在三种族人群中,白介素4(C 590T)基因型及等位基因与哮喘、哮喘严重度无相关性(P>0.05)。然而,华人的白介素 4(T590)等位基因(98.3%)显著高于马来人(84%)及印度人(35.9%)(P<0.01)。【结论】华人、马来人及印度人的白介素4(C 590T) 基因多态性与哮喘无关。然而,此基因多态性存在种族差异。更多还原

【Abstract】 To investigate the association between IL-4 promoter gene (IL-4 C-590T) polymorphisms and asthma in Chinese, Malay and Indian.Three hundred and fifteen asthmatics (Chinese 152; Malay 76; Indian 87) and 360 healthy blood donors (Chinese 157; Malay 100; Indian 103) were recruited. The patients were subdivided into two groups: mild to moderate asthma group [Forced expiratory volume in one second (FEV1)≥ 60% predicted] and severe group (FEV1 <60% predicted). IL-4 C-590T polymorphisms were determined by PCR-RFLP (restriction fragments length polymorphisms). A logistic regression model was applied to detect the association between asthma and IL-4 C-590T polymorphisms. The significant level was set as P<0.05.Our data showed that there was no significant association between IL-4 C-590T polymorphisms and asthma, asthma severity. However, in the control group, the prevalence of IL-4 T590 allele in Chinese (98.3%) was significantly higher than that of Malay (84%) and Indian (35.9%) (P<0.01).[Conclusion]There is no significant association between IL-4 C-590T and asthma in Chinese, Malay and Indian. However, IL-4 C-590T shows ethnic variations.更多还原