【摘要】 目的:观察雷公藤内酯醇(Triptolide,Tri)对体外培养的牛晶状体上皮细胞(bovine Lens epithelial cell,BLECs)增殖细胞核抗原的影响和对BLECs增殖的作用。方法:取第4代对数生长期的牛LECs,培养24h后,加入重组人表皮生长因子(Recombinant Human Epithelial Growth Factor,rhEGF)(终浓度50μg/L)和不同浓度的Tri(40,20,10μg/L),再分别作用6,12,24,48,72h后,采用流式细胞仪检测增殖细胞核抗原(PCNA)在LECs的阳性表达率及增殖抑制率。结果:不同浓度Tri(40～10μg/L)抑制处于增殖状态的LECs,PCNA表达率在6～72h内随浓度增高和作用时间延长有明显的下调,10μg/L组6hPCNA表达是(76.74±0.8),与同时间增殖对照组比较有显著性差异(P<0.05),12h(70.32±1.6),24h(61.96±2.1),48h(54.98±2.0),72h(42.15±1.6);20μg/L组6hPCNA表达是(68.58±3.2),12h(55.34±2.6),24h(49.59±1.6),48h(36.61±0.5),72h(30.00±1.1);40μg/L组6hPCNA表达是(60.96±1.2),12h(46.45±1.7),24h(32.10±0.5),48h(27.15±1.1),72h(19.16±1.5),以上各组与同时间增殖对照组比较均有非常显著性差异(P<0.01),呈明显的时间—效应关系和剂量—效应关系。结论:雷公藤内酯醇使牛晶状体上皮细胞内PCNA的表达率下降,改变细胞周期,从而抑制细胞增殖。更多还原

【Abstract】 Objective: To determine the effects and mechanisms of the Triptolide on antigen expression of proliferation cell nuclear from bovine lens epithelial cells (BLECs) in vitro. Methods: The fourth generation of incubated BLECs were cultured for 24 hours with the recombinant human Epithelial Growth Factor (rhEGF)of 50 μg/L and Triptolide was added in different concentrations(40,20,10 μg/L)for 6～72 hours 6,12, 24, 48 and 72 h respectively. The expression rates of proliferating cell nuclear antigen (PCNA) were measured with FCM(Flow Cytometry) in order to observe and determine the inhibitory effects of Triptolide on the proliferation of BLECs. Results: With the different concentrations of Triptolide (40～10 μg/L)- the expressions of PCNA 6～72 h showed down regulation following the higher concentrations and the longer time. The expression of PCNA in 6,12,24,48,72 h at the concentration of 10 μg/L group were (76.46±0.8),(70.32±1.6),(61.96±2.1),(54.98±2.0), (42.15±1.6) respectively comparing with the proliferation groups at the same time- the differences were significant. P<0.05. There were (68.58±3.2),(55.34±2.6),(49.59±1.6),(36.61±0.5),(30.00±1.1) in the same time interval at the concentration of 20 μg/L group; and(60.96±1.2),(46.45±1.7),(32.10±0.5),(27.15±1.1),(19.16±1.5) at the concentration of 40 μg/L group respectively. Comparing with other proliferation groups of the same time interval- there were great significant differences P<0.01-which showed both time and dose dependent relations. Conclusion: Triptolide decreasesexpression rate of PCNA- changes the cell cycle and inhibits effectively the proliferation of BLECsin vitro.更多还原