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食管癌组织中线粒体DNA突变检测

Detection of mitochondrial DNA mutations in esophageal cancer tissue

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【Author】 SONG Qian 1) , ZHANG Shijie 2) , WANG Mingchen 3) , GAO Li 4) , WANG Zhiju 5) , ZHAO Guoqiang 2) , DONG Ziming 1)# 1)Department of Pathophysiology,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 2)Department of Microbiology and Immunology,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 3)Department of Biochemistry,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 4)Department of Pediatrics,Henan Provincial People′s Hospital, Zhengzhou 450003 5)Department of Physiology,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052

【机构】郑州大学基础医学院病理生理学教研室；郑州大学基础医学院微生物学与免疫学教研室；郑州大学基础医学院生物化学教研室；河南省人民医院儿科；郑州大学基础医学院生理学教研室；郑州大学基础医学院病理生理学教研室郑州450052；郑州450052；郑州450003；郑州450052；

【摘要】目的:检测食管癌组织中线粒体DNA(mtDNA)的突变。方法:提取8例食管癌组织的mtDNA;PCR扩增mtDNA的控制区,纯化PCR产物、与T载体连接,转化大肠杆菌,筛选出阳性克隆,用引物T7/SP6做PCR鉴定后测序,与GenBank序列对比。结果:8例标本mtDNA的控制区共有36个位点发生突变,其中转换32个,颠换4个;同义突变13个,错义突变23个。结论:mtDNA突变很可能在食管癌的发生中发挥一定作用。更多还原

【Abstract】 Aim: To detect the mitochondrial DNA(mtDNA) mutations in esophageal cancer tissue. Methods: mtDNA was extracted from tissue of 8 patients with esophageal cancer. D-loop region was amplified using PCR. The PCR products were purified, and linked with T-vector and transformed into JM109. The positive clone was screened and identified by primer T7/SP6 and then sequenced. The mtDNA mutations in the tumor tissue were compared with the GenBank sequence. Results: Mutations were found in all specimens.Totally 36 sites of mutation were found,including 32 sites of coversion,and 4 sites of transversion;13 sites of synonymous mutation and 23 sites of missense mutation. Conclusion: mtDNA mutation probably plays a role in the development of esophageal cancer.更多还原

【关键词】食管肿瘤；线粒体DNA； D-loop区；突变；
【Key words】 esophageal neoplasm； mitochondrial DNA； D GAAB2 loop region； mutation；

【基金】国家自然科学基金资助项目39870287

【文献出处】郑州大学学报(医学版) ,Journal of Zhengzhou University(Science Medical) , 编辑部邮箱 ,2005年04期

【分类号】R735.1
【下载频次】137

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