【摘要】 目的:研究人B7-2基因修饰的食管癌细胞作为瘤苗的抗肿瘤作用。方法:将真核荧光表达载体pEGFP-C3-B7-2,通过脂质体转染技术转染人食管癌细胞株EC9706。外周血来源的树突状细胞(DC)负载肿瘤抗原后,与自体T淋巴细胞共培养3d,获得细胞毒性T淋巴细胞(CTL)。用四甲基偶氮唑蓝(MTT)法检测CTL对转染和未转染pEGFP-C3-B7-2的人食管癌细胞EC9706的杀伤活性。结果:CTL对转染pEGFP-C3-B7-2的肿瘤细胞的杀伤活性大于转染pEGFP-C3和未转染细胞的杀伤活性(P<0.05)。结论:人B7-2基因修饰的EC9706肿瘤细胞瘤苗,可诱导出明显的抗EC9706细胞的免疫效应。更多还原

【Abstract】 Aim: To study the immunological effect of esophageal cancer cell vaccine modified by human B7-2 gene. Methods: Human esophageal cancer cell line EC9706 was transfected with the vector of pEGFP-C3-B7-2 uging the technique of lipofectamine transfection. The dendritic cells(DCs) induced from peripheral blood mononuclear cells(PBMC) were loaded with the tumor antigen, and co-cultured with congeneric T cells derived from PBMC for 3 days,to obtain the tumor specific cytotoxic T lymphocytes(CTL). Methyl thiazolyl tetrazolium(MTT) assay was used to detect inhibition effect of CTL on transfected and untransfected EC9706 cells. Results: CTL had stronger inhibition response to the cancer cells transfected with pEGFP-C3-B7 GAAB2 2 than to the cancer cells transfected with pEGFP-C3 and the untransfected cancer cells (P< 0.05).Conclusion: The tumor vaccine modified by human B7-2 gene can significantly induce response to kill esophageal cancer cells.更多还原