【摘要】 目的:探讨氦氖激光穴位照射疗法对缺血缺氧新生大鼠脑损伤后海马神经元增殖的影响。方法:7d龄健康Wistar大鼠72只随机分成4组:假手术组、缺血缺氧组、缺血缺氧激光穴位照射组、缺血缺氧激光非穴位照射组。制备新生大鼠缺血缺氧脑损伤模型,激光穴位照射组选择“百会”和“大椎”穴位给予氦氖激光照射,激光非穴位照射组选择腹部非穴位部位进行激光照射,常规饲养22d后处死,处死前经腹腔注射5溴2脱氧脲嘧啶(BrdU)标记增殖细胞。取左侧脑做组织切片,HE、Nissl染色以及采用抗BrdU抗体和神经上皮蛋白(Nestin)抗体进行免疫组织化学染色。结果:激光穴位照射组与其他组比较海马神经元胞体内尼氏体丢失较少,神经元坏死减轻,海马齿状回BrdU标记的免疫阳性细胞数增加,海马CA1区Nestin免疫阳性细胞数增高。结论:激光穴位照射对海马神经元有保护作用,促进了海马神经元的增殖。更多还原

【Abstract】 Aim: To study the effect of the proliferation on hippocampal neuron with He-Ne laser acupoint irradiation after hypoxia-ischemia in neonatal rats.Methods: Seven-day-old Wistar rats (n=72) were randomly divided into four groups as sham-operatin group, hypoxia-ischemia group, He-Ne laser acupoint irradiation group and He-Ne laser unacupoint irradiation group. The neonatal rats were prepared animal model of unilateral hypoxia-ischemia.The laser acupoint irradiation group were irradiated on“Baihui”and “Dazhui” points synchronously,the unacupoint irradiation group were irradiated on abdominal unacupoint synchronously. The rats were decapitated after HIBD 22 days.BrdU was injected into the abdominal cavity of each rat to label the proliferating cells. The left tissue of brain was regularly paraffin wax embedded, sectioned and stained by HE staining. Nissl staining, BrdU and Nestin immunohistochemical staining were performed. Results: The number of Nissl bodies lost was decreased in hippocampus, BrdU and Nestin positive neurons increased obviously in hippocampal in He-Ne laser acupoint irradiation group. Conclusion: He-Ne laser acupoint irradiation has neuroprotective effect against hypoxia-ischemia and promotes the proliferation of hippocampal neuron after hypoxia-ischemia in neonatal rat brain.更多还原