【摘要】 目的:探讨 8 -Br- cAMP对人视网膜母细胞瘤HXO Rb44细胞系的凋亡效应。方法:以 8-Br- cAMP体外作用HXO -Rb44细胞,分为 3个实验组和 3个对照组,分别进行 24h, 48h和 72h培养。应用RNA斑点印迹、免疫组化斑点印迹及原位杂交技术,分别对HXO -Rb44细胞的bcl 2杂交信号和PCNA、Fas、FasL表达信号进行检测。采用TUNEL法检测HXO -Rb44细胞的凋亡率。结果:在不同培养时间的实验组中HXO -Rb44细胞的凋亡率明显高于各自的对照组(未经 8- Br- cAMP处理);而PCNA、Fas、bcl- 2杂交信号扫描数值低于对照组,FasL杂交信号扫描数值高于对照组,尤以 48h的作用最显著。结论: 8- Br cAMP可能是通过下调细胞内的bcl -2基因、Fas和PCNA的表达和上调FasL的表达而诱导HXO- Rb44细胞系凋亡。更多还原

【Abstract】 Aim: To study the apoptotic effects of 8-Br-cAMP on human retinoblastoma HXO-Rb44 cells.Methods: The HXO-Rb44 cells were cocultured with 8-Br-cAMP for 24 h, 48 h, and 72 h, respectively. There were 3 experimental groups incubated with 8-Br-cAMP and 3 control groups treated without 8-Br-cAMP. The expression of bcl-2 and expression of Fas, FasL, and PCNA were detected by in situ hybridization, immunohistochemistry,and RNA immuno-dot blot techniques. The cell apoptosis was assayed by TUNEL method. Results: The cell apoptostic rate markedly increased in all the experimental groups. The TLC scanning values of Bcl-2, Fas, and PCNA signals in the 3 experimental groups were lower than those of control groups, while FasL signals in the 3 experimental groups were higher than those of control groups,especially in 48 h group.Conclusion: 8-Br-cAMP can induce apoptosis through down-regulating the expression of bcl-2, Fas,and PCNA, and up-regulating the exprestion of FasL in human HXO-Rb44 cells.更多还原