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食管癌淋巴结转移组织中COL21A1,RUNX2,COAS基因定量检测

Detection of COL21A1, RUNX2, and COAS gene in SCC metastatic lymph nodes by RQ-PCR

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【Author】 QIN Yanru 1,2), WANG Lidong 2) , ZHUANG Zehao 3), KUANG Liyun 4), GUAN Xinyuan 4), JORGE Tsao 5), SI Huaxin 5) 1)Department of Oncology, the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 2)Laboratory for Cancer Research, College of Medicine, Zhengzhou University, Zhengzhou 450052 3)Department of Gastroenterology, the First Affiliated Hospital,Fujian Medical University, Fuzhou 350005 4)Department of Clinical Oncology Queen Mary Hospital, Hong Kong 5)Department of Anatomy, Hong Kong University, Hong Kong

【机构】郑州大学第一附属医院肿瘤科；郑州大学癌症研究室；福建医科大学附属第一医院消化内科；香港大学玛丽医院临床肿瘤学系；香港大学解剖学系；香港大学解剖学系郑州450052郑州大学癌症研究室郑州450052；郑州450052；福州350005；

【摘要】目的:探讨 3种可能的食管癌转移相关基因COL21A1、RUNX2和COAS与食管癌淋巴结转移的相关性。方法:选择 4例经病理证实为食管鳞状细胞癌伴淋巴结转移病例,应用实时荧光定量PCR(RQ- PCR)技术测量其淋巴结转移灶中COL21A1、RUNX2和COAS基因的扩增倍数。结果:COL21A1扩增倍数分别为 2. 50, 31. 34, 0. 68,6. 45;RUNX2扩增倍数分别为 4. 44, 1. 29, 0. 23, 3. 25; COAS扩增倍数分别为 2. 11, 10. 13, 0. 44, 4. 99。3种基因在3 /4食管癌转移淋巴结组织中表达增强。结论:COL21A1,RUNX2和COAS基因可能与食管癌的淋巴结转移有关。更多还原

【Abstract】 Aim: To explore the relationship between the expressions of COL21A1, RUNX2 and COAS gene and the metastasis of esophageal squamous cell carcinoma.Methods: Four cases of metastatic lymph nodes tissue of esophageal cancer were subjected to quantity COL21A1, RUNX2 and COAS gene expression by RQ-PCR.Results:Compared with β-actin, the relative gene expression values of COL21A1 in the 4 samples were 2.50, 31.34, 0.68 and 6.45,respectively; while those of RUNX2 were 4.44, 1.29, 0.23, 3.25, and 2.11, 10.13, 0.44 and 4.99 for COAS gene,respectively.The expression of the 3 genes were higher in 3/4 samples.Conclusion: COL21A1, RUNX2 and COAS gene may be related with the lymph node metastasis of esophageal squamous cell carcinoma.更多还原

【关键词】 COL21A1； RUNX2； COAS； RQ-PCR；食管肿瘤；淋巴结；
【Key words】 COL21A1； RUNX2； COAS； RQ-PCR； esophageal neoplasm； lymph node metastasis；

【基金】国家杰出青年科学基金资助项目　30025016;河南省医学科技创新人才工程项目　2003 26

【文献出处】郑州大学学报(医学版) ,Journal of Zhengzhou University(Science Medical) , 编辑部邮箱 ,2005年01期

【分类号】R735.1
【被引频次】1
【下载频次】171

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