【摘要】 目的:研究幽门螺杆菌超声粉碎抗原与霍乱毒素B亚单位组成的口服疫苗预防Hp感染的作用,探讨其保护性免疫应答的机制。方法:用Hp超声粉碎抗原2mg加CTB 10 μg作为免疫预防组,另设单纯Hp超声粉碎抗原组、单纯CTB组和PBS组为对照组。免疫4w后以活菌攻击,观察各组小鼠的免疫保护率,小鼠胃粘膜分泌性IgA抗体变化情况。结果:(1)免疫预防组小鼠免疫保护率为73.33%和对照组全部感染Hp(P <0 .0 0 1) ;(2 )免疫后4w各组小鼠未见明显的炎症反应;Hp攻击感染后4w免疫预防组小鼠可见明显的炎症反应,而各对照组相对较轻(P <0 .0 1) ;(3)Hp攻击前后免疫预防组小鼠局部胃粘膜IgA抗体显著升高(P <0 .0 1)。结论:Hp超声粉碎抗原加CTB可预防Hp感染。更多还原

【Abstract】 Objective To investigate the effects of the oral vaccin e containing Helicobacter pylori (H.pylori)sonicate plus cholera toxin B subu nits(CTB) in the prevention of H.pyloriinfection in mice,and study the prot ective immune resp onse induced by the vaccine.Methods Mice were orally immunized by H.pyloriso nicate 2mg plus CTB 10μg.Mice immunized with H.pylorisonicate,CTB alone or PBS were used as control. Four weeks after immunization, mice were challenged with H .pylori.The protective rate after challenge,serum anti-H.pyloriand secr etory I gA in gastric mucosa in different groups were studied before and after challenge . Results (1)Protective rate of the immunized group was 73.33%, while the contro l groups were all infected(P<0.001). (2)In protected mice, severe gastritis was noted at four weeks after challenge,compared with the gastritis seen in control groups(P<0.01). (3)The immunized group had a significant higher level of sec reto ry IgA in gastric mucosa before and after challenge(P<0.01).Conclusi on The oral vaccine containing H.pylorisonicate plus CTB is effective in preventing fro m H.pyloriinfection in mice.更多还原