【摘要】 目的:探讨徒手连续气液交换应用于复杂玻璃体视网膜显微手术(VRMS)术后良好效果的机制。方法:对71例复杂视网膜脱离手术成功的患者行2~3次徒手连续气液交换,将前2次取到的玻璃体腔再形成液作用于离体培养的人视网膜色素上皮细胞(RPE),研究其对RPE细胞分泌bFGF的作用及对视网膜RPE细胞凋亡基因bcl-2和Ki-67表达的影响。结果:玻璃体腔再形成液作用于RPE细胞爬片后可使其Bcl-2和Ki-67表达上调以及bFGF分泌增加。结论:徒手连续气液交换可机械性减少玻璃体视网膜术后眼内的细胞增殖因子,间接抑制RPE的增殖,提高玻璃体视网膜手术的成功率。更多还原

【Abstract】 Objective To demonstrate the mechanism of positive effects of the sequential air-fluid exchange on the use of complicated vitroretinal microsurgery. Methods Seventy-one patients who underwent vitreoretinal microsurgery were performed 2 or 3 times sequential air-fluid exchange. The regenerated fliud in vitreous cavity at various periods was collected to act on cultured human retinal pigment epithelial (RPE) cells,and then the secretion of bFGF and the expressions of bcl-2 and ki-67 by RPE cells were observed. Results The expressions of Bcl-2 and ki-67 were up-regulated and the secretion of bFGF significantly increased after RPE cells was acted with the regenerated fluid in the vitreous cavity. Conclusion The sequential air-fluid exchange can mechanically reduce intraocular growth factors after the vitreoretinal microsurgery, indirectly restrain the proliferation of RPE cell, and improve the successful rate of vitreoretinal microsurgery.更多还原