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两种基因型泡桐体细胞胚胎发生及植株再生

SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION OF DIFFERENT GENOTYPES OF Paulownia PLANTS

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【作者】 范国强翟晓巧马新业黎明

【Author】 FAN Guo-qiang ZHAI Xiao-qiao MA Xin-ye LI Ming(Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan, 450002)

【机构】 河南农业大学泡桐研究所河南农业大学泡桐研究所 河南郑州450002河南郑州450002现在河南省林业科学研究院河南郑州450002河南郑州450002

【摘要】 以叶片和茎段为外植体,研究了毛泡桐和白花泡桐胚性愈伤组织诱导、体细胞胚胎发生及植株再生情况。结果表明,白花泡桐叶片和茎段胚性愈伤组织诱导的最适培养基分别为MS+0.3mgLNAA+14mgLBA和MS+0.3mgLNAA+8mgLBA,其最适培养基皆为MS+0.3mgLNAA+17mgLBA。从体细胞胚胎发育所需时间来看,两种泡桐叶片的体细胞胚胎发育能力均高于茎段。

【Abstract】 The induction of embryogenic calli, somatic embryogenesis and plantlet regeneration from leaves and nodal segments of two genotypes of paulownia plants were studied. The results indicated that the optimal media of embryogenic callus induction from leaves and nodal segments of P. fortunei were MS+03mg/L NAA+14mg/L BA and MS+03mg/L NAA+8mg/L BA respectively,and those media of embryogenic callus induction were MS+03mg/L NAA+ 17mg/L BA. The capability of somatic embryogenesis from leaves of two genotypes of paulownia plants was higher than that from the nodal segments.

【基金】 国家自然科学基金(批准号30271082)
  • 【文献出处】 核农学报 ,Acta Agriculturae Nucleatae Sinica , 编辑部邮箱 ,2005年04期
  • 【分类号】S792.43
  • 【被引频次】36
  • 【下载频次】187
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