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中华鳖种群RAPD分析
RAPD Analysis of Genomic DNA in the Pelodiscus Sinensis
【摘要】 选用14个随机引物对中华鳖基因组DNA进行了随机扩增DNA片段多态性(RAPD)分析.14个引物,在8个待检样品中共扩增出714条清晰的DNA片段,片段大小在200~3 000 bp之间,平均每一引物在每个样品中扩增了6.0条带.统计结果表明,中华鳖群体内平均相似率0.857,总平均带纹频率0.773,遗传变异率为0.227.研究表明,中华鳖为遗传共享度较高的群体.
【Abstract】 The genomic DNA of the Pelodiscus sinensis were ampilified with 14 random primers,then 714 bands were observed with 14 primers in 8 samples.The length of these DNA fragments were varied from 200 to 3 000 bp.On average,there existed about 6.0 bands being observed with single primer and each sample.The intra-population band sharing was 0.857,average band frequency was 0.773,and the genetic variability was 0.227.
- 【文献出处】 湖南师范大学自然科学学报 ,Journal of Natural Science of Hunan Normal University , 编辑部邮箱 ,2005年03期
- 【分类号】S917.4;
- 【被引频次】13
- 【下载频次】148