【摘要】 采用高效液相色谱二极管矩阵检测器测定了乌索酸的紫外光谱,建立了分析色谱峰纯度的方法.采用傅立叶红外变换光谱仪测定了乌索酸的红外光谱,得到乌索酸的特征 IR光谱结构,解析了乌索酸的 IR光谱与其结构特征的关系,确定了乌索酸 IR光谱的结构特征和官能团归属.IR光谱的谱峰形态清晰可辨,使乌索酸的主要结构和官能团均能得到准确的分析和指认,与质谱联用,推定了乌索酸的分子骨架、官能团位置和结构.更多还原

【Abstract】 In this article, HPLC with a photodiode array detector was used to record the UV spectra of the ursolic acid. The analysis method of chromatogram peak has been established. Fourier transform-infrared spectroscopy (FTIR) was used to record the IR spectra of the ursolic acid and get the IR spectral characteristics of the ursolic acid. The relationship of the wave peaks of IR spectra and the structural characteristics was analyzed. The attribution of the functional groups in the structural characteristics of ursolic acid was basically defined. The peaks in the IR spectra are clear and distinguishable and are used to assign the functional groups easily. Using with mass spectrum together, molecule framework, position of functional groups and structure of ursolic acid is concluded.更多还原