【摘要】 为建立良好的刺葡萄原生质体分离体系,利用原生质体融合技术对刺葡萄进行品种改良,以刺葡萄叶片、根尖和愈伤组织为材料,研究了经不同酶液组合和酶解时间处理,再经过0.45μm筛网过滤,1000r/min低速离心后分离产生原生质体的情况.试验结果表明,原生质体分离材料以愈伤组织最好,叶片次之,根尖最差;4次以内继代培养的愈伤组织,经2%纤维素酶+0.5%果胶酶+1%离析酶的酶液组合酶解8h后,原生质体的产量为5.12×106个,活力为88.57%.更多还原

【Abstract】 To establish a system for protoplast isolation, and improve the character with the technology of protoplast fusion of Vitis davidii Fo?x,by way of using its blade,root tip and different callus as the materials,the yield and viability of protoplast was measured,through different combination of enzyme,different dissolved times,adoption of the 0.45 μm screen to filtrate,and then the separation at low-speed of 1000 r/min.The results show that the most suitable material is callus for protoplast separation,the second is blade,root tip is the worst.The optimised method for callus was to subculture it in no more than 4 times and dissolve it 8 hours with a combination of 2% cellulase + 0.5% pectinase +1% macerating enzyme,then the yield of protoplast can be up to 5.12×106,the viability can be up to 88.57%.更多还原