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5株传染性支气管炎病毒分离株的鉴定和血清分型

Identification and serotyping of five infectious bronchitis virus isolates

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【作者】 陈峰吴孔兴张齐冯寿华张秀丽廖秋生曹永长

【Author】 CHEN Feng~1,WU Kong-xing~2,ZHANG Qi~2,FENG Shou-hua~2,ZHANG Xiu-li~2,LIAO Qiu-sheng~2,CAO Yong-chang~1(1 College of Animal Science,South China Agric.Univ.,Guangzhou 510642,China; 2 Guangdong Wen’s Foodstuff Group,Xinxing 527439,China)

【机构】 华南农业大学动物科学学院广东温氏食品集团有限公司华南农业大学动物科学学院 广东广州510642广东新兴527439广东广州510642

【摘要】 从四川、重庆和广东3个不同地区,不同时间发病的肉鸡中分离出5株病毒.通过鸡胚发育试验、NDV干扰试验、动物回归试验和感染后鸡血清中抗体水平变化确定这5株病毒均为传染性支气管炎病毒.血清中和试验表明,这5株病毒血清型与北京分离的A2株一致,而与H52、H120、M41、T和Holte株不同.该研究结果证实了鸡传染性支气管炎病毒类4/91毒株在我国华南和西南地区的流行.

【Abstract】 Five field strains of infectious bronchitis virus(IBV) were isolated from the affected broilers in Chongqing City、Sichuan and Guangdong Provinces by inoculation into allantoic cavity of 10-day SPF embryos.Biological characteristics of the isolates were studied by virus pathogenicity to SPF chicken embryos and chickens,hemagglutination activity of virus to chicken red blood cells,interference with NDV and detection of antibody titer of post-infected chickens.The results revealed that all of the five stains were IBV.Moreover,the data of viral-neutralization indicated that the serotypes of the five strains were similar to IBV strain A2,but different from classic IBV strains H52,H120,M41,T and Holte.The results provided foundation for prevention of infectious bronchitis in these areas.

【基金】 “十五”国家重点攻关计划(2001BA508B21);广东省科技攻关计划重大项目(2004A20106001)
  • 【文献出处】 华南农业大学学报 ,Journal of South China Agricultural University , 编辑部邮箱 ,2005年04期
  • 【分类号】S852.65;
  • 【被引频次】13
  • 【下载频次】210
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