【摘要】 采用离子交换凝胶QSepharoseFastFlow,以NaCl梯度洗脱,将紫球藻胞外多糖 (ESPS)分离成3个组分ESPS0、ESPS1.0和ESPS1.6,分别占粗多糖总量的44.2%(以质量 计,下同),20.3%和16.4%.ESPS0中未测出蛋白,ESPS1.0和ESPS1.6的蛋白含量分别为 7.8%和7.6%;ESPS0、ESPS1.0和ESPS1.6的硫酸基含量分别为16.3%,11.6%和8.3%.利 用体外模型测试了3个组分的抗CVB3病毒的活性.发现ESPS0,ESPS1.0和ESPS1.6抑制 CVB3病毒的半数抑制质量浓度分别为2.03,0.51和0.53mg/L,治疗指数IT值分别为 61.6,245.1和235.8;在相同条件下,阳性对照药物病毒唑的半数抑制质量浓度和IT值 分别为31.25mg/L和16.0.结果表明,3种组分抗CVB3活性均高于病毒唑.更多还原

【Abstract】 Three components, named ESPS₀, ESPS 1.0 and ESPS 1.6, were separated from extracellular polysaccharide （ESPS） of Porphyridium sp. by NaCl gradient elution with Q Sepharose Fast Flow gel. ESPS₀, ESPS 1.0 and ESPS 1.6 account for 44.2% （in mass, the same below）, 20.3% and 16.4% of the total ESPS, their protein content were at 0, 7.8% and 7.6%, SO₃- radical content were 16.3%, 11.6% and 8.3%, respectively. By in vitro anti-CVB₃ activity test to the three components, the results show that their mass concentrations of half inhibition to virus were at 2.03, 0.51 and 0.53mg/L, and I_T values were at 61.6, 245.1 and 235.8, respectively. Under the same conditions, the mass concentration of half inhibition to virus and I_T value of the positive control drug Ribavirin were at 31.25mg/L and 16.0. They indicate that the anti-CVB₃ activity of all the three components were higher than Ribavirin.更多还原