【摘要】 研究了在培养液中添加不同浓度的EGF、IGF-1以及EGF联合IGF-1对山羊有腔卵母细胞体外成熟的影响。结果表明: (1)10, 20, 30μg/LEGF对山羊卵母细胞体外成熟无显著影响(P>0.05)。(2) 10μg/L的IGF-1对山羊卵母细胞体外成熟无显著影响(P>0.05); 20, 30μg/L的IGF-1能显著提高山羊卵母细胞体外成熟率 (P<0.05)。(3)添加 10μg/LEGF+20μg/LIGF-1组卵母细胞体外成熟率显著高于添加 20μg/L的IGF-1组(P<0.05),极显著高于添加 10μg/LEGF组和对照组(P<0.01)。可见,EGF和IGF-1对山羊卵母细胞体外成熟有协同作用。更多还原

【Abstract】 Three Experiment were conducted to determine the effect of EGF and IGF-1 on in vitro maturation of goat oocytes. Oocytes were cultured in Oocytes Maturation medium with EGF and IGF-1 alone or combinedly at different concentration. The results in experiment 1 showed that there was no significant different between control group and the group with EGF(10μg/L、20μg/L、30μg/L) in maturation rate. In experiment II the maturation rate of goat oocytes was improved significantly in the groups with EGF(20μg/L、30μg/L) (P<0.05),but not in the group with 10μg/L EGF. In experiment III the maturation rate in the group containing 10μg/L EGF and 20μg/L IGF-1 was significantly higher than 20μg/L IGF-1 and 10μg/L EGF groups.The results obtained in this study indicate that EGF and IGF-1 have coeffects on in vitro maturation of goat oocytes.更多还原