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产氢菌的16S-23S rDNA间隔区的长度变异性分析(英文)
Sequence length variation of internal genic space of 16S rDNA-23S rDNA in biohydrogen-bacterium
【摘要】 生物制氢细菌Rennanqilyf3的16SrRNAgene(rDNA)-23SrDNA间隔区碱基序列被测定.利用PCR扩增间隔区DNA,间隔区碱基序列存在长度多态现象.用这一长度多态现象进行产氢发酵细菌的辨认和识别.产氢发酵细菌Rennanqilyf3的16SrRNAgene(rDNA)-23SrDNA间隔区的PCR产品从1270到398bp,共有5个序列.碱基数目分别为1270、398、638、437和436bp.
【Abstract】 A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic regions was developed for the identification of species for fermentative biohydrogen-producing bacterium. The sizes of the PCR products varied from 1 270 to 398 bp. Strain of Rennanqilyf3 were characterized as having products of 1 270,398,638, 437 and 436bp.
【关键词】 生物制氢;
产氢细菌;
16S23SrDNA基因间隔区;
长度变异性;
【Key words】 biohydrogen; H2-producing bacterium; 16S-23S rDNA intergenic region; length variation;
【Key words】 biohydrogen; H2-producing bacterium; 16S-23S rDNA intergenic region; length variation;
【基金】 国家杰出青年科学基金(50125823).
- 【文献出处】 哈尔滨商业大学学报(自然科学版) ,Journal of Harbin University of Commerce(Sciences Edition) , 编辑部邮箱 ,2005年03期
- 【分类号】X172
- 【被引频次】4
- 【下载频次】116