【摘要】 目的探讨视神经损伤后的自然修复再生和脑源性神经营养因子及睫状神经营养因子互补辅助再生的图形视觉诱发电位变化特征,寻找视神经有效再生的新途径。方法用猫作为实验对象,术前动物随机分为四组:正常对照组、球后视神经半径截断组、视神经半径截断并定时玻璃体内注射生理盐水对照组和视神经半径截断并定时玻璃体内注射脑源性神经营养因子和睫状神经营养因子复合因子辅助再生组。经眶外侧入路手术开眶行视神经半径截断术。术后四组动物共同在正常视环境中饲养1、4和8个月,复合因子辅助再生组在术后即日注射脑源性神经营养因子和睫状神经营养因子复合液(10 ng),并在以后2次/周注射等量脑源性神经营养因子和睫状神经营养因子复合液。在视神经损伤后1、4和8个月,3次行视神经半径动态检测。结果在视神经半径截断损伤后1个月时,与正常对照组相比,视神经半径损伤各组的图形视觉诱发电位N1-P1振幅显著降低(P<0.001),P1潜时延迟(P<0.001);视神经半径截断组和生理盐水对照组与复合营养因子辅助再生组比较,图形视觉诱发电位的N1-P1振幅也出现了降低和P1潜时的延迟(P均<0.01)。在视神经半径损伤后第4个月时,视神经半径截断组和视神经半径切断并定时玻璃体内注射生理盐水对照组与正常对照组比较,其图形视觉诱发电位的P1潜时显著延迟(P均<0.001)和N1-P1幅度显著降低(P均<0.001)。而复合因子辅助再生组与正常对照组比较图形视觉诱发电位的P1潜时具有显著性(P<0.05),但与视神经损伤后1个月时同组动物的图形视觉诱发电位测试结果比较出现了恢复型曲线图谱并差异有显著性(P<0.01)。在视神经损伤8个月后,视神经截断组和生理盐水对照组图形视觉诱发电位的P1潜时还是显著延迟和N1-P1幅度显著降低,未见明显的恢复型曲线图出现。复合因子辅助再生组图形视觉诱发电位的P1潜时和N1-P1幅度与视神经损伤后4个月时图形视觉诱发电位测试的结果比较差异具有显著性(P<0.01),与视神经半径截断组和生理盐水对照组比较差异具有显著性(P均<0.01),与正常对照组比较其图形视觉诱发电位的P1潜时未见延迟(P>0.05),但N1-P1幅度还低于正常对照组(P<0.05)。结论动态观察图形视觉诱发电位变化结果说明,1/2损伤的视神经在复合神经营养因子的互补作用下可促进其修复再生和恢复视觉信息的传导功能。更多还原

【Abstract】 Objective To investigate the effect of neurotrophins(BDNF and CNTF) in repairment and regeneration of optic nerve(ON) after hemi-injury.To search a new way for functional and effective optic nerve regeneration.Methods Cats were randomly divided into 4 groups,10 cats were used as normal control group(N);10 cats for ON1/2 semidiameter incision group(ON1/2I);10 cats as ON1/2 incision and physiological saline injection group(ON1/2I+PS);10 cats as ON1/2 incision,ciliary neurotrophic factor(CNTF) and brain-derived neurotrophic factor injection group(BDNF),(ON1/2I+BF).After the experimental cats were operated the animals were fed for four weeks,sixteen weeks and thirty-two weeks respectively under the same condition of visual environment.N,ON1/2I,ON1/2I+PSW and ON1/2I+BF were examined with pattern visual evoked patential(PVEP) for three times(in the 1st,4th,8th month).Then,the results of PVEP were analysed and investigated with two pair T-test.Results At the first month after the optic nerve was injured at 1/2 semidiameter,compared with N,PVEP of N1-P1 amplitude lowered and P1 latency delayed for all of ON1/2 injured groups.Also,compared with ON1/2I+BF,PVEP of N1-P1 amplitude lowered and P1 latency delayed for ON1/2I and ON1/2I+PS.At the fourth month after the optic nerve was injuried at 1/2 diameter,ON1/2I and ON1/2I+PS were compared with N for N1-P1 amplitude and P1 latency of PVEP,there was no significant difference.Also,their N1-P1 amplitude and P1 latency of PVEP were lowered and delayed compared with ON1/2I+BF.In addition,when ON1/2I+BF was compared with N,there was also significant difference in the N1-P1 amplitude and P1 latency of PVEP.But the curve lines of the N1-P1 amplitude and P1 latency of PVEP of ON1/2I+BF showed recovery compared with the results at the first month.At the eighth month after the optic nerve was injured at 1/2 of diameter,compared with N, PVEP of N1-P1 amplitude was lowered and P1 latency delayed in ON1/2 and ON1/2I+PS.The curves of the N1-P1 amplitude and P1 latency of PVEP for ON1/2 and ON1/2I+PS had no recovery compared with the rerults at the first month.Also,there was no significant difference bewteen ON1/2I+BF and N for PVEP of P1 latency.But N1-P1 amplitude was droped compared with that of N(P<0.05).However,there was also significant difference(P<0.01) when ON1/2I+BF was compared with ON1/2 and ON1/2I+PS.There was significant difference(P<0.01) bewteen ON1/2I+BF in the eighth month and in the fourth month for PVEP of N1-P1 amplitude and P1 latency.Its curve lines of the N1-P1 amplitude and P1 latency of PVEP at the eighth month showed recovery.Conclusion Injured ON recoveries function of conduction for visual information and has an effective regeneration through the effect of compound of BDNF and CNTF.更多还原