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脑得生片中葛根素的含量测定
Improvement of Naodesheng Tablets Determination Method
【摘要】 目的:建立脑得生片中葛根素的含量测定方法。方法:采用超声波提取,以高效液相色谱法测定葛根素的含量,流动相为甲醇—水(25:75),流速1ml·min-1,柱温25℃,检测波长250nm。结果:葛根素在0.5016~4.5144μg 范围内呈良好的线性关系,回归方程为 Y=37527300X+49230,r=0.9999;平均回收率为100.91%(RSD1.10%),重复 RSD 为1.92%。结论:该方法准确、快捷、重复性好。
【Abstract】 Objective:To develop a method for the determination of puerari in Naodesheng tablets.Methods:Uses the ultrasonic wave to withdraw.Determination of Puerari content by HPLC.The mobile phase is the methanol-water(25: 75),speed of flow 1 ml.min-1,column temperature 25℃,examination wave length 250nm.Results:The calibration curve was in good linearity over the range of 0.5016~4.5144μg,the regression equation is Y=37527300X+49230,r=0. 9999;The average returns-ratio is 100.91%(RSD 1.10%),duplicated RSD is 1.92%.Conclusion:The method is accu- rate,quick,the duplication is good.
- 【文献出处】 黑龙江医药 ,Heilongjiang Medical Journal , 编辑部邮箱 ,2005年03期
- 【分类号】R286
- 【被引频次】8
- 【下载频次】64