【摘要】 目的研究类毒素鄄A(ANTX鄄A)对PC12细胞内ATP酶活力的影响。方法用不同浓度(0、10-9、10-8、10-7mol/L)ANTX鄄A刺激PC12细胞1h,或10-7mol/LANTX鄄A刺激PC12细胞不同时间(0、30、60、90min)后,诱导PC12细胞激活;2相刺激方法(用ANTX鄄A2次处理PC12细胞)诱导PC12细胞脱敏;用比色法测定这2种状态下胞内Na+鄄K+鄄ATP酶和Ca2+鄄ATP酶活力。结果10-9、10-8、10-7mol/LANTX鄄A激活PC12细胞1h和10-7mol/LANTX鄄A激活PC12细胞30、60、120min时,胞内Na+鄄K+鄄ATP酶活力均有下降趋势,Ca2+鄄ATP酶活力在不同激活状态均比对照组显著降低(P<0.05,P<0.01),有明显的剂量鄄反应关系和时间鄄效应关系。在2相脱敏试验中,当第1相ANTX鄄A浓度为10-8、10-7mol/L时,所对应脱敏状态胞内Na+鄄K+鄄ATP酶活力也均有减少趋势,Ca2+鄄ATP酶活力比相应激活状态明显降低(P<0.01)。结论Ca2+鄄ATP酶在ANTX鄄A激活和脱敏PC12细胞过程中可能有重要调节作用。更多还原

【Abstract】 Objective To study the effects of anatoxin-a (ANTX-A) on the activity of ATPase in PC12 cells.Methods PC12 cells were activated by different concentrations of ANTX-A stimulation for an hour or 10-7 mol/L ANTX-A for different time.PC12 cells were desensitized using twice incubation method.The activities of Na+-K+-ATPase and Ca2+-ATPase were determined by colorimetry method.Results 10-9,10-8,10-7 mol/L ANTX-A activated PC12 cells for an hour and 10-7 mol/L ANTX-A activated PC12 cells for 30,60,120 min,the activity of Na+-K+-ATPase in PC12 cells,in all of the cases,showed an reduction tendency,the activity of Ca2+-ATPase decreased significantly in different activation situation compared to the control group(P<0.05,P<0.01 respectively),and had a dose-time effect relation.During the assay of twice incubation desensitization,as the fist incubation concentration of ANTX-A was 10-8 or 10-7 mol/L,the activity of Na+-K+-ATPase still had a decrease tendency in each desensitization status,the activity of Ca2+-ATPase reduced significantly compared to the control group (P<0.01).Conclusion Ca2+-ATPase may play an important role in the activation and desensitization of PC12 cells induced by ANTX-A.更多还原