【摘要】 目的研究四氯二苯并二叶恶英(TCDD)诱导HepG2肝癌细胞、肺癌A549、SPC鄄A1细胞的细胞凋亡。方法分别用0.01、0.10、1.00μmol/LTCDD对3种细胞染毒24h,再细胞爬片和HE染色,光学显微镜下观察细胞形态。结果细胞凋亡可在0.01、0.10、1.00μmol/LTCDD处理后24h出现。表现为细胞固有形态丧失,核染色质固缩、碎裂,出现凋亡小体,并随染毒浓度加大而更明显。SPC鄄A1细胞的凋亡率分别为9.56%,16.8%,21.7%;A549的凋亡率分别为7.7%,13.6%,19.4%;HepG2的凋亡率分别为8.5%,14.5%,30%,均高于对照组,3组癌细胞凋亡率与对照组相比,差异有统计学意义(P<0.05)。结论TCDD可能诱导此3种细胞发生凋亡。更多还原

【Abstract】 Objective To investigate apoptosis of three kinds of carcinoma cells (HepG2,lung cancer A549 and human lung carcinoma cells SPC-A1) induced by TCDD(2,3,7,8-tetrachlorodibenzo-p-dioxin).Methods After three kinds of carcinoma cells were treated with TCDD for 24 h,the slides with covered with cells were prepared and stained with HE,the morphological changes of cells were observed under microphotography.Results The apoptosis appeared after 24 h of treatment with 0.01,0.10,1.00 μmol/L TCDD,intrinsic morphological feature,nuclear chromatin withered and broke,the apoptotic bodies could be noticed in apoptosis cells.These changes became prominent as TCDD concentration increases.The apoptosis percentages of SPC-A1 were 9.56%,16.8% and 21.7%,that of A549 were 7.7%,13.6% and 19.4%,that of HepG2 were 8.5%,14.5% and 30%,they were significantly higher compared with the control.Conclusion TCDD can induce apoptosis in three kinds of cells,HepG2,A549 and SPC-A1.更多还原