【摘要】 目的研究有机磷农药——氧乐果对小鼠睾丸特征性酶的影响以及茶多酚对它的拮抗作用。方法将80只昆明雄性小鼠随机分成8组,即3个氧乐果染毒组,1个对照组,1个茶多酚组和茶多酚+低、中、高剂量氧乐果染毒组。染毒组分别灌胃1、2、4mg/kg氧乐果;茶多酚组灌胃180mg/kg茶多酚;茶多酚+低、中、高剂量染毒组小鼠提前灌胃茶多酚180mg/kg,2h以后分别灌胃1、2、4mg/kg氧乐果,对照组灌胃生理盐水,共灌胃6d。用比色法检测睾丸组织中睾丸特征性酶——碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)及乳酸脱氢酶X酶(LDH-x)的活力。结果随着氧乐果染毒剂量的增加,小鼠体重和睾丸重下降(P<0.01或P<0.05),睾丸AKP、ACP和LDH活力均升高(P<0.05或P<0.01)。预先灌胃茶多酚,2h后灌胃氧乐果,小鼠体重和酶活力有所变化,但与对照组比较,差异均无统计学意义(P>0.05)。结论茶多酚对氧乐果所造成的小鼠睾丸特征性酶的影响有拮抗作用。更多还原

【Abstract】 Objective To study the effect of omethoate on the activity of characteristic enzymes in mouse testicles and the antagonism of teapolyphenol. Methods 80 Kunming male mice were divided into 8 groups, omethoate was given by gavage: the control group given 0.9% NaCl solution, a group given teapolyphenol of 180 mg/kg bw, three groups given omethoate of 1, 2 and 4 mg/kg bw respectively, three groups given teapolyphenol of 180 mg/kg bw and two hours later followed by omethoate of 1, 2 and 4 mg/kg bw respectively. The gavages were conducted for 6 consecutive days. The activities of the characteristic enzymes (AKP, ACP, LDH and LDH-x) in mouse testicles were measured by biochemistry method and variance analysis was done with the SPSS 11.0 software. Results As omethoate dose increased, the body weight and the testicle weight decreased obviously, the activities of AKP, ACP, LDH in mouse testicles significantly increased compared with the control. Given teapolyphenol 2 hours before given omethoate, the significant changes as above did not emerged. Conclusion Teapolyphenol has an antagonism against the adverse effects of omethoate on the activity of characteristic enzymes in mouse testicles.更多还原