【摘要】 目的研究以甲醇和汽油为燃料的汽车尾气(以下简称甲醇车尾气和汽油车尾气)对小鼠RAW264.7细胞的细胞毒性及对其增殖和凋亡的影响。方法用甲醇车尾气和汽油车尾气对RAW264.7细胞染毒,用四甲基偶氮唑盐(MTT)比色法确定受试物的染毒剂量。RAW264.7细胞分甲醇车尾气组(分别用0.0078、0.0156、0.03125、0.0625、0.125L/ml的甲醇车尾气染毒)和汽油车尾气组(染毒剂量与甲醇车尾气组相同),并分别设空白对照组。分别对各个组进行乳酸脱氢酶(LDH)活力测定、细胞形态学观察、检测细胞周期并计算细胞增殖指数及计算凋亡率。结果在MTT试验中,汽油车尾气组在0.125、0.5、1、2、4L/ml剂量组的光密度(OD)值低于对照组(P<0.01),甲醇车尾气组在0.5、1、2、4L/ml剂量组的OD值低于对照组(P<0.01)。汽油车尾气组在0.1250.0625、0.03125L/ml剂量组的LDH活力高于对照组(P<0.05);汽油车尾气组0.03125、0.0625L/ml剂量组和甲醇车尾气组0.0625L/ml剂量组的细胞增殖指数低于对照组(P<0.05);甲醇车尾气组和汽油车尾气组在0.0156、0.03125、0.0625L/ml剂量组凋亡率均高于对照组(P<0.05)。结论同等剂量下甲醇车尾气的细胞毒性明显大于汽油车尾气;汽油车尾气和甲醇车尾气对RAW264.7细胞株凋亡的影响相近;汽油车尾气对细胞增殖的抑制作用略大于甲醇车尾气。更多还原

【Abstract】 Objective To study the cytotoxicity and the adverse effects of gasoline exhaust and methanol exhaust on proliferation and apoptosis of RAW264.7 cells. Methods The cytotoxicity was detected by MTT assay. RAW264.7 cells were divided into two groups, gasoline vehicle exhaust exposure group and methanol vehicle exhaust exposure group. The samples of gasoline and methanol exhaust were added to the culture medium at concentration of 0, 0.007 8, 0.015 6, 0.031 25, 0.062 5 and 0.125 L/ml respectively. LDH activity in medium was analyzed. Cytomorphology was observed. The cell proliferation index and cell apoptosis rate of different exposure groups were determined by flowcytometry. Results In group of gasoline, there was significant lower OD values at 0.125 L/ml and above compare with the control (P<0.01). In group of methanol, there was significant lower OD values at 0.5 L/ml and above compare with the control(P<0.01). In group of gasoline, there was higher LDH values at 0.031 25-0.125 L/ml compared with the control (P<0.05). Compare with the control, the cell proliferation index at 0.031 25, 0.062 5 L/ml in the group of gasoline and at 0.015 6, 0.031 25, 0.062 5 L/ml in the group of methanol decreased significantly (P<0.05), the percentage of apoptosis cell at 0.015 6, 0.031 25, 0.062 5 L/ml in both the group of gasoline and the group of methanol increased significantly (P<0.05). Conclusion The cytotoxicity of gasoline exhaust to RAW264.7 cells is distinctly stronger than that of the methanol exhaust. As for the apoptosis, gasoline exhaust and the methanol exhaust showed the same adverse effects. For proliferation inhibit, the effect of gasoline exhaust is slightly greater than methanol exhaust.更多还原