【摘要】 目的测定复方补骨脂酊中补骨脂素和异补骨脂素的含量。方法采用YWGC18色谱柱(4.6mm×200mm),流动相为54%甲醇(含1%冰醋酸溶液),流速为0.7ml/min,检测波长为295nm。结果补骨脂素和异补骨脂素保留时间分别为9.165min和10.082min,标准曲线的线性范围均为20～200mg/l(补骨脂素r=0.9999;异补骨脂素r=0.9993)。补骨脂素和异补骨脂素中的高、中、低、三种浓度溶液的日间和日内变异均小于10.0%,加样回收>90%。测定复方补骨脂酊中补骨脂素和异补骨脂素的含量分别为2.058、2.270、2.201mg/ml和1.665、1.617、1.609mg/ml。结论本法简便、快速重现性好,可用于复方补骨脂酊制剂质量控制的含量测定方法。更多还原

【Abstract】 Objective HPLC method for determination of the contents of psoralen and isopsoralen angelicin in compound Buguzhi tincture was employed.Methods The HPLC analysis was made on a column of YWG C18(4.6 mm×200 mm)with a mobile phase of 54% methanol(1% acetic acid glacid solution contained).The detection wavelength was at 295 nm and the flow rate was 0.7 ml/min.Results The retention time of psoralen and isopsoralen angelicin were 9.165 min and 10.082 min respectively.The linearity range of psoralen(r=0.9999)and isopsoralen angelicin(r=0.9993) were between 20 to 200 mg/l.The with-day and between-day RSD for psoralen and isopsoralen angelicin at different concentration was less than 10.0% with average recovery rate more than 90%.The content of psoralen and isopsoralen angelicin in compound Bugnzhi tincture was 2.058,2.270,2.201 mg/ml and 1.655,1.617,1.609 mg/ml respectively.Conclusion The method is simple and reproducible and may be taken as the quality control for compound Buguzhi tincture.更多还原