【摘要】 研究了酰胺类物质对促进变性牛碳酸脱水酶(CAB)的复性作用,考察了在较低盐酸胍浓度下添加剂浓度对复性收率的影响,并利用三级反应动力学方程对酰胺类化合物辅助CAB复性的动力学过程进行了深入分析.结果表明,含有酰胺基团的多种添加剂均能有效促进CAB复性;酰胺化合物与盐酸胍的作用相似,即复性速率常数kN值均大于无添加剂时的自发复性,且随添加剂浓度增加而降低;但聚集体生成速率常数kA值则在最佳添加剂浓度下出现最小值,从而使kN/kA值最大,复性收率最大.更多还原

【Abstract】 The effect of various amides on the refolding of denat ur ed carbonic anhydrase B (CAB) was investigated at low guanidine hydrochloride (G dmCl) concentration.The competitive model of first-order folding and third-ord er aggregation was found to well describe the dynamic refolding process in the p resence of the additives. The results indicated that the denatured CAB could be properly refolded in the presence of the amides, and the effect of the amides on assisting CAB refolding was found kinetically similar to that of GdmCl. The ref olding rate constant k_N decreased with increasing additive concentration, while there was an optimum additive concentration where a minimum aggregation ra te constant k_A was obtained. At this additive concentration, the value of the ratio of k_N to k_A was maximum, leading to the maximal refolding yield of CAB.更多还原