【摘要】 筛选到一株产氨基酰化酶的真菌刺孢小克银汉霉9980,直接利用其菌体细胞拆分N乙酰DL苯丙氨酸,最终制得D苯丙氨酸。最适反应条件为:0.2mol/LN乙酰DL苯丙氨酸(含Co2+5×10-4mol/L),0.04g/mL菌体,在pH值为7.0、50℃条件下反应24h,拆分率达90%以上。产物经JK008阳离子交换树脂分离。N乙酰D苯丙氨酸经6mol/LHCl加热回流4h脱乙酰得D苯丙氨酸。DPhe[α]20D=+34.4°(水溶)。更多还原

【Abstract】 An enzymatic method for the production of D-phenylalanine by aminoacylases from Cunnighamella echinulata 9980 was developed. The optimum conditions were as follows: 0.2 mol/L N-Ac-DL-phenylalanine（including Co 2+ 5×10 -4mol/L）,0.04 g/mL mycelia, pH 7.0. After 24 h incubation at 50 ℃,the conversion rate against N-Ac-L-phenylalanine was higher than 90%. L-phenylalanine and N-Ac-D-phenylalanine were separated and purified on JK008 ion exchange resin. N-Ac-D-phenylalanine was hydrolyzed by 6 mol/L hydrochloric acid for 4 h, then D-phenylalanine crystal was obtained. D-Phe[α] 20_D=+34.4°（H₂O）.更多还原