【摘要】 目的:研究抑癌基因 p16 蛋白在脑胶质瘤发生、分化过程中的作用及其与预后的关系。方法:本院1999年6月至2002年6月接受手术及术后立体定向放射治疗68例脑胶质瘤患者,应用免疫组化的方法检测其病理标本中的p16蛋白,分析p16蛋白的缺失与病理分级、患者预后之间的关系。结果:全组共有37例(54.4%)标本p16蛋白表达阴性,Ⅰ、Ⅱ、Ⅲ、Ⅳ级胶质瘤的 p16 蛋白表达阴性率分别为14.3%、33.3%、66.7%、75.0%,Ⅲ、Ⅳ级胶质瘤的p16蛋白的阴性率明显高于Ⅰ、Ⅱ级(P<0.01)。p16蛋白表达阴性患者的复发率明显高于p16蛋白表达阳性者(P<0.01),分别为 54.5%和 19.4%。结论:p16蛋白的缺失与脑胶质瘤的恶性程度有关,恶性程度越高,p16 蛋白的缺失率越高;p16蛋白的缺失与预后相关,其缺失率越高,患者复发率越高。更多还原

【Abstract】 Objective: To investigate the correlation of p16 expression with the proliferation, malignant progression and prognosis of brain glioma. Method:68 patients of brain glioma were operated with stereotactic radiotherapy after operation from June 1999 to June 2002. Express of p16 protein of the specimens was detected by immunohisto- chemistry. The relationship between the expression of p16 protein and the pathological grade and the recurrence of the tumor were analyzed. Result: There were 37cases (54.4%) that the expression of p16 protein was positive. The positive rate of Ⅰ、Ⅱ、Ⅲ、Ⅳ pathological grade was 14.3%、33.3%、66.7% and 75.0% respectively. The positive rate of Ⅲ and Ⅳ grade was significantly higher than Ⅰand Ⅱ grade.(P<0.01) The recurrence rate of patients with negative expression of p16 protein (54.5%)was significant higher than the those with positive negative expression(19.4%). Conclusion: The loss or mutation of p16 gene is one of the reasons of the malignant progression in brain glioma. The immunohistochemical detection of p16 protein acts as a prognosis index for brain glioma.更多还原