【摘要】 目的:探讨苯并[a]芘(BaP)作用下HSP70、P53在人胚肺细胞(HEL)中的定位及共表达。方法:培养正常人胚肺二倍体细胞,分别以0,10,50,100,200μmol·L-1BaP染毒3h,用双重免疫荧光组织化学法和激光扫描共聚焦显微镜检观察HSP70、P53的表达。结果:HEL细胞经不同浓度的BaP染毒3h后,HSP70在胞浆、胞核均有表达,随BaP染毒浓度的增加,逐渐由细胞浆表达向核转移;而P53表达在胞浆、胞核均呈增加趋势(除200μmol·L-1BaP外),胞核中P53蛋白表达水平略高于胞浆;小于50μmol·L-1BaP处理的HEL细胞,HSP70和P53以细胞浆共表达为主,而BaP浓度大于50μmol·L-1时,主要表现为胞核共表达。结论:BaP染毒的人胚肺细胞HSP70、P53的分布及定位方式具有浓度依赖的趋势,表现为随BaP染毒浓度的增加,HSP70与P53由胞浆共表达转变胞核共表达。更多还原

【Abstract】 Objective: To investigate the localization and coexpression of heat s hock protein 70 (HSP70) and P53 in the human embryonic lung (HEL) cells treated by benzo(a)pyrene (BaP). Methods: HEL cells were cultured and t reated at different concentrations of BaP (0, 10, 50, 100, 200 μmol·L~-1) for 3 hours. The expressions of P53 and HSP70 were detected by immunohistochemi stry and Confocal Laser Scanning Microscopy technology. Results: The expression of HSP70 was detected in both the nucleus and cytoplasm in BaP\ |treated HEL cells, and the expression mainly concentrated in the nucleus when t he concentration of BaP was increasing. A similar distribution of P53 protein wa s observed in BaPstimulated HEL cells, however, the levels of P53 in the nucle us were higher than that in the cytoplasm. The coexpression of HSP70 and P53 w as found in the cytoplasm at the dose of less than 50 μmol·L~-1, and in t he nucleus at the dose of more than 50 μmol·L~-1 BaP. Conclusion : The immunolocalition and distribution of HSP70 and P53 in HEL cells t reated by benzo(a)pyrene(BaP) is in a dosedependent manner, and the localizati on of coexpression of HSP70 and P53 is changed from the cytoplasm to the nucle us as BaP concentration increasing.更多还原