【摘要】 目的:制备pcDNA3.1(+)gbpB壳聚糖纳米基因防龋疫苗,并检测其经鼻免疫大鼠的效果。方法:制备两种pcDNA3.1(+)gbpB壳聚糖纳米颗粒载体系统,经鼻粘膜免疫SD大鼠,用ELISA法检测血清中针对葡聚糖结合蛋白B的特异IgG抗体滴度及唾液中针对葡聚糖结合蛋白B的特异SIgA抗体滴度。与阳性对照组霍乱毒素pcDNA3.1(+)gbpB及未免疫组、空白壳聚糖纳米颗粒组作比较。结果:该载体系统经鼻免疫SD大鼠所产生的血清及唾液中针对GbpB的IgG和SIgA抗体滴度远大于未免疫组和空白壳聚糖纳米颗粒组,而与阳性对照组霍乱毒素pcDNA3.1(+)gbpB组抗体滴度相当。且维持的抗体滴度更稳定和持久。结论:成功构建了以葡聚糖结合蛋白B基因为免疫原的纳米基因防龋疫苗,并获得较好的免疫效果。更多还原

【Abstract】 Objective: To examine the effect of intranasal administration of pcDNA3.1(+)-gbpB-chitosan nanoparticles on the systemic and mucosal immune response. Methods: Two formulations of pcDNA3.1(+)-gbpB-chitosan nanoparticles were prepared and administrated to immunize Sprague-Dawley(SD) rats through the nasal. Serum and salivary samples were collected periodically, GbpB-specific IgG and IgA antibodies were measured by an adapted method enzyme-linked immunosorbent assay (ELISA). Results: The system and local immune response were significantly higher than that observed as the negative controls. Conclusion: The chitosan nanoparticles formulated in this study were suitable for the intranasal anti-carious plasmid DNA vaccine delivery.更多还原