【摘要】 以IBH120鸡胚毒适应成纤维细胞(CEF)单层,培养出IBH120细胞弱毒株。电镜观察,可见典型的冠状病毒粒子。IBH120细胞弱毒株经细菌、霉菌、支原体、鸡白血病病毒检测均为阴性,且具有IB病毒特异性。CEF单层接毒后48h左右病毒增殖达到高峰。随着细胞传代次数的增加,C4~C10代H120株细胞毒的毒价(TCID50)逐步增高而趋于稳定,C4~C10代细胞毒的TCID50稳定在107.35~8.0mL之间,而C5~C10代IBH120细胞毒的EID50呈下降趋势,变动范围在107.3~106.5mL。以103.5TCID50/只和104.5TCID50/只的剂量,C4,C8,C10代毒分别滴鼻接种3日龄敏感鸡,免疫鸡的攻毒保护率为80%~100%,病毒分离回收率分别为20%和0。更多还原

【Abstract】 Cellular attenuated strain of infection bronchitis H₁₂₀ strain(IBH₁₂₀)was cultured successfully with virus which cultured on the chicken embryo.Atypical Coronary viral partical were observed by electric microscope.IBH₁₂₀ cellular attenuated strains were negative after examination bacterium,mildew,mycoplasma,chicken leukemia virus,moreover,they speciticity of IB virus.Virus proliferate could reach peak after 48 h.When CEF cells infected with IBH₁₂₀,TCID₅₀ of C₄-C₁₀ generation of H₁₂₀ strain cellular attenuated virus rose gradually and stabilized with increasing generation.TCID₅₀ of C₄-C₁₀ generations were between 10^7.35 and 10^8.0.but EID₅₀ of C₅-C₁₀generation were degressive trend.The variational range was between 10^7.3 and 10^6.5.Theimmunogenicity of IBH₁₂₀ cellular attenuated virus was checked.The result demonstrated that three days old chickens were inoculated in nose with 10^3.5 TCID₅₀ and 10^4.5 TCID₅₀ of C₄,C₈,C₁₀ generation virus in each chicken respectively,their protection rate was between 80% and 100%.The virus separation recovery rate was 20% and 0 respectivelly.更多还原