节点文献
不同激素浓度对柠条茎段组织培养及植株再生的影响
Effects of Different Plant Hormone Concentrations on Tissue Culture and Plantlet Regeneration of Caragan microphylla
【摘要】 以小叶锦鸡儿茎段为外植体,MS为基本培养基,设计不同浓度的6-BA,IAA,NAA,IBA,KT等单因子和不 同比例的细胞生长素和细胞分裂素组合试验。结果表明:在芽的诱导增殖过程中,添加有6-BA 0.5 mg/L,IAA 0.01 mg/L的MS培养基效果最好,诱导率为90%。在生根培养过程中,添加有IAA 0.5 mg/L的1/2MS培养基效果较 好,生根率为86%。
【Abstract】 Stalk segments of Caragan microphylla Lam were cultured as explants on medium supplemented with plant hormone in different concentrations. The results showed that Murashige and Skoog(MS) medium supplemented with 6-BA 0.5 mg/L,IAA 0.01 mg/L was the most effective medium for the differentiation and multiplication of the shoots. The optimum rooting medium was 1/2MS + IAA 0.5 mg/L.
【关键词】 柠条;
组织培养;
植株再生;
激素;
【Key words】 Caragana microphylla Lam; Tissue culture; Plantlet regeneration; Plant hormone;
【Key words】 Caragana microphylla Lam; Tissue culture; Plantlet regeneration; Plant hormone;
【基金】 国家863项目(2002AA241019)部分内容
- 【文献出处】 华北农学报 ,Acta Agriculturae Boreali—Sinica , 编辑部邮箱 ,2005年01期
- 【分类号】S793.3
- 【被引频次】13
- 【下载频次】198