【摘要】 对猪血中超氧化物歧化酶(SOD)的纯化工艺进行了研究。首先将红细胞中加入Triton X-100溶液,用冻融的方法使细胞膜破裂溶血。然后依次用有机溶剂乙醇-氯仿去除血红蛋白、磷酸盐萃取、丙酮沉淀和热处理的方法进行初步纯化得到粗酶液,最后上DEAE Sephadex A-50层析柱,经PAGE电泳检测,得到的SOD达到电泳纯,酶的比活力为5863 U.mg-1,回收率达到61%。更多还原

【Abstract】 Superoxide dismutase（SOD） was purified from porcine erythrocyte in this paper.First,erythrocyte membrances were disintegrated via freeze-thraw methods in the presence of Triton X-100.Second,porcine erythrocyte coarse Cu·Zn-SOD was obtained by several steps,which were about removing hemoglobin by ethanol and chloroform firstly,extraction by dipotassium hydrogen phosphate secondly,precipitation by acetone thirdly,heat denaturation fourthly,etc.In the end,through DEAE Sephadex A-50,it was completely purified.The yield and the specific activity of SOD after this process were 61% and 5863 U·mg^-1,respectively.Purified SOD was testified by PAGE electrophoresis.更多还原