【摘要】 低能离子对细胞表面的刻蚀和溅射作用为外源 DNA的进入提供了通道,同时离子注入又对宿主细胞的DNA产生一定的损伤,这就有可能通过选择不同的离子、离子能量和剂量实现外源DNA在受体细胞中的转导。初步论述了离子束介导外源基因转导的基本原理及其在植物、微生物转基因的应用。更多还原

【Abstract】 Ion implantation may echo or bore on the surface of cell,thus provide convenience for exogenous macromolecule transferring into cell. Ion at certain low energies with appropriate doses can increase permeability of the cell wall and induce exogenous macromolecule transferring. In this paper, we summarize recent advances in research on low energy ion beam mediated genetic transferring mechanism and applications in plant and microorganism.更多还原