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红花长寿花茎段及叶片的离体培养与快速繁殖

Study on in vitro culture and micropropagation from the stem sections and t he leaves of Kalanchoe blossfeldiana with red flower

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【作者】 林秀莲赖钟雄黄双龙吴金寿黄玉吉黄晓霞柯存祥

【Author】 LIN Xiu-lian, LAI Zhong-xiong , HUANG Shuang-long, WU Jin-shou, HUANG Y u-ji, HUANG Xiao-xia, KE Cun-xiang (Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry Univ ersity, Fuzhou, Fujian 350002, China)

【机构】 福建农林大学园艺植物生物工程研究所福建农林大学园艺植物生物工程研究所 福建福州350002福建福州350002福建福州350002

【摘要】 采用红花长寿花茎段和叶片作为外植体进行离体培养与快繁研究。结果表明,茎段和带叶柄的叶片可以直接诱导出芽;在MS+0.25mg·L-1NAA+1mg·L-1BA+2mg·L-1GA培养基上有利于腋芽诱导和丛生芽形成;在1/2MS+0.1mg·L-1NAA培养基中生根效果最好。

【Abstract】 Stem sections and leaves from the plants of Kalanchoe blossfeldiana with re d flower were used as explants for in vitro culture and micropropagation. Th e result showed that buds were directly induced from the stem section and the le aves with leafstalks; and the MS medium supplemented with 0.25 mg·L -1NAA, 1 mg·L -1BA and 2 mg·L -1GA was beneficial to the induction of axil lary buds and the formation of bud clusters; and the highest percentage of rooti ng was obtained on the 1/2 MS medium adding 0.25 mg·L -1NAA .

【关键词】 红花长寿花茎段叶片离体培养
【Key words】 Kalanchoe blossfeldianastem sectionleafin vitro culture
【基金】 国家“948”计划资助项目(No.991029)
  • 【文献出处】 亚热带农业研究 ,Sugarcane , 编辑部邮箱 ,2005年02期
  • 【分类号】S682.3
  • 【被引频次】10
  • 【下载频次】340
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