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氦-氖激光诱变细菌细胞及原生质体选育果胶酶高产菌株

The Breeding of High-Yield Pectinase Producing Bacteria Using He-Ne Laser Irradiation on the Cell and Protoplasts

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【Author】 Zhu Hongli 1,2,Guo Ailian1,Song Jirong2,Yang Xiufang3,Zhang Jia1,Zhang Xiaorui1 1 Institute of Life science,Northwest University,Xi′an 710069 2 Institute of chemistry Engineering,Northwest University,Xi′an 710069 3 College of Machinery and Precision Instrument,Xi′an University of Technology,Xi′an 710048 Received date:20050401

【机构】西北大学生命科学学院；西北大学化工学院；西安理工大学机械与精密仪器工程学院；西北大学生命科学学院西安710069西北大学化工学院；西安710069；西安710048；西安710069；

【摘要】利用氦氖激光(波长632.8nm,功率15mW)诱变果胶酶产生菌的菌体细胞,获得了突变株ZHg,其酶活达到301u·mL-1,是出发菌株的1.3倍.进一步用氦氖激光照射ZHg的原生质体,从而得到了高产突变株ZHgA,其酶活达到357u·mL-1,比ZHg提高了18%,是出发菌株的1.6倍.该菌株在好氧和静置条件下均能良好产酶,经传代实验证明其产酶性能稳定.更多还原

【Abstract】 A mutant strain ZH-g was screened by He-Ne laser mutagenesis on the cells.Its pectinase activity was in 1.3 fold of the original one,reaching 301 μ/mL.On the basis of this,using He-Ne laser irradiating on the protoplasts of ZH-g,the mutant strain ZH-gA with high yield of pectinase was obtained.It accumulated pectinase activity up to 357 μ/mL which was 18% higher than that of the parent strain.This strain can produce pectinase efficiently under both aerobic and static cultivation.The enzyme activity is stable after several times of batch.更多还原

【关键词】果胶酶；细菌； He-Ne激光；原生质体；诱变；
【Key words】 Pectinase； Bacteria； He-Ne laser； Protoplast； Mutagenesis；

【基金】陕西省教委基金资助项目(00JK138)

【文献出处】光子学报 ,Acta Photonica Sinica , 编辑部邮箱 ,2005年11期

【分类号】TQ925.3;
【被引频次】31
【下载频次】212

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